Catalase and hydrogen peroxide experiment coursework

Once one of both compounds is depleted, the product formation stops. This damage is a natural result of reactions happening inside your cells. If collecting the gas over water is complicated, and you have access to a cm3 gas syringe, you could collect the gas in that instead.

In fact, the catalase reaction is dependent on the substrate concentration. The time taken to fit into and leave the active site is the limiting factor in the rate of reaction. Set out in brown bottles Note 1.

Science Coursework. Line of best fit - hydrogen Peroxide and Catalase?

Implementation of any Science Project Idea should be undertaken only in appropriate settings and with appropriate parental or other supervision. I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks.

Ask students to explain how values over 22 cm3 could happen. It does so according to the following reaction: Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide.

Values below 22 cm3 show that oxygen has escaped, or the hydrogen peroxide has not fully reacted, or the hydrogen peroxide concentration is not as expected.

Take care when removing the cap of the reagent bottle, as gas pressure may have built up inside.

Exploring Enzymes

Wash splashes of pureed potato or peroxide off the skin immediately. If collecting the gas over water is complicated, and you have access to a cm3 gas syringe, you could collect the gas in that instead.

Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously. Before using the hydrogen peroxide, put on your safety goggles to protect your eyes.

Cleanup Pour all the solutions into the sink and clean all the spoons with warm water and dish soap. I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate.

Though in theory, this should be the trend, my results did not demonstrate this pattern. This is shown when oxygen stops being produced and the same results are recorded five times.

However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions. Repeat the catalase reaction but this time vary conditions such as the pH by adding vinegar an acid or baking soda a baseor change the reaction temperature by heating the solution in the microwave.

To prevent this I had to dry out the barrel and syringe before commencing the procedure. Put the syringe in place in the bung of the flask, but do not push the plunger straight away. As I have mentioned earlier, this decreases in steps of 3.

Use a cm3 measuring cylinder for concentrations of hydrogen peroxide over 20 vol. Background Enzymes are essential for our survival.

What happens if you keep the substrate concentration constant but change the concentration of the enzyme? Do not return solution to stock bottles, because contaminants may cause decomposition and the stock bottle may explode after a time.

This is because at higher temperatures, molecules of both enzyme and substrate have more kinetic energy and collide more often. Liver also contains catalase, but handling offal is more controversial with students and introduces a greater hygiene risk.

This heat energy is transferred to the environment.

Effect of Substrate Concentration on the Rate of Activity of Catalase

The oxygen produced in 30 seconds is collected over water. This may have been because I only used a pipette when measuring the hydrogen peroxide, and poured the water into the volumetric flask to make up the rest of the cm3.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

This easy test, which can be seen with the naked eye, without the aid of instruments, is possible because catalase has a very high specific activitywhich produces a detectable response, as well as the fact that one of the products is a gas. Each chemical reaction needs a minimum amount of energy to make it happen.

A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction.

However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.Catalase breaks down the hydrogen peroxide into water and oxygen before it damages the cell (Frederick, ).

The purpose of our lab is to prove [1] how catalase speeds up the break down of hydrogen peroxide and determine the reaction rate at which it does [2].

When the enzyme catalase comes into contact with its substrate, hydrogen peroxide, it starts breaking it down into water and oxygen. Oxygen is a gas and therefore wants to escape the liquid. Experiment 6A Biology with Calculators 6A - 1 Enzyme Action: Testing Catalase Activity Many organisms can decompose hydrogen peroxide (H 2O 2) enzymatically.

Enzymes are globular proteins, responsible for most of the. Published: Wed, 23 May The investigation was carried out to determine how different ph levels and hydrogen concentrations, affect the activity of catalase in potato during the decomposition of hydrogen peroxide to produce water and oxygen.

- The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen by using the enzyme catalase.

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Catalase and hydrogen peroxide experiment coursework
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